Biogenic Synthesis Of Chitosan Silver Nanocomposites Using Mud Crab (Scylla Serrata) And Its Anti-Microbial And Anti-Cancer Studies
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Abstract
hitosan is a marine polysaccharide commonly seen in the crustacean family, and the exoskeleton is covered by chitosan. In this study, shells of mud crabs were subjected to chitosan extraction which has been followed by demineralization, deproteination, and deacetylation. The chemical compositions of chitosans were characterized using Fourier transform infrared spectroscopy (FTIR). It exhibits antimicrobial activity against pathogens -E.coli, Staphylococcus, Klebsiella pneumonia, Bacillus cereus, and Salmonella typhi. It has therefore received attention as a potential food preservative. Transform Infrared Spectroscopy (FTIR) also spots out chemical bonds in a molecule by creating rather an infrared absorption spectrum. FTIR is an effective instrument for analysis and for also detecting the functional groups as well as characterizing covalent bonding information. FTIR spectroscopy finds out the functional molecules involved in the synthesis of nanoparticles and also, provides accuracy and reproducibility, which helps to detect small absorbance changes in the sample. The chemical compositions of chitosans were characterized further using FESEM.The FESEM pattern performs or indicates the silver nanoparticles to be embedded in a matrix of CS. The size of the particles is seen as with their uniformity. Not only this, but also the FESEM image of Ag nanoparticles shows somewhat the particles of spherical shape. Also, by Transmission Electron Microscopy (TEM) technique, the chemical compositions of chitosans were characterized. It exhibits that synthesized chitosan/silver nanocomposites have indeed a shape such as spherical with their uniformity as to their dispersion. Further, the chemical compositions of chitosans were characterized using Electron dispersive spectroscopy (EDS). The EDS histograms reveal rather successfully the percentage weight ratios of the elements found on the chitosan surface and the nanosilver concentrations in this study. In addition, by XRD analysis the chemical compositions of chitosans were characterized. It recognizes the multifarious crystalline phases present in the material so that the information touching the chemical compositions are revealed. In this study, mcf-7 cell line is chosen. Mcf-7 is a commonly used breast cancer cell line. For evaluation of anticancer study against mcf-7, the mcf-7 cell line is chosen for the study. In this study, anti-cancer study was very specially subjected to research and the chemical compositions of chitosans were characterized very seriously by FESEM, TEM, EDS, FTIR, and XRD for such anticancer study and therapy.
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