In Vitro And In Vivo Pharmacological Evaluation Of Mimosa Pudica Leaf And Root Extract For Antinephrolithiatic Activity
Main Article Content
Abstract
The growth of crystals in the urinary tract is known as urolithiasis. Mimosa pudica L. is used as a diuretic and to cure kidney stones in Ayurveda and other traditional medicine systems. The goal of the current investigation was to assess the anti-urolithiatic activity of Mimosa pudica L. extracts in rats that had been experimentally induced to develop urolithiatic behaviour, despite the lack of scientific evidence to support this activity.
Method- Sodium oxalate incited urolithiasis model in the rodent was utilized to survey the impact of oil ether remove Mimosa pudica. The examination is intended to discover the impact of concentrates of Mimosa pudica on restorative utilization against sodium oxalate incited urolithiasis.
Group Treatment (Root Extract )
Group-1 Receive Saline (1 ml/kg)
Group-2 Receive Sodium oxalate (7mg/100g,in)
Group-3 Receive Sodium oxalate+vehicle (7 mg/100g, ip)
Group-4 Receive Sodium oxalate+Cystone (7 mg/100g, ip + 500mg/kg,p).
Group-5 Receive Sodium oxalate+ Ether extract. (250mg/kg)
Group-6 Receive Sodium oxalate+Ether extract. (500mg/kg)
Group-7 Receive Sodium oxalate+ Ethanolic extract. ( 250mg/kg)
Group-8 Receive Sodium oxalate +Ethanolic extract. (500 mg/kg)
Group-9 Receive Sodium oxalate +Aqueous extract. ( 250 mg/kg)
Group – 10 Receive Sodium oxalate +Aqueous extract. (500 mg/kg)
All rodents was housed in metabolic pens independently for the whole term of the investigation. The urine of each rodent was gathered on seventh day after 6 hrs of sodium oxalate infusion with Thymol as an (additive) and serum of each rodent would be gathered. Assessment of biochemical boundaries viz Urea, Uric corrosive, Creatinine, Sodium, Chlorides and Potassium in serum and urine will be finished. Helpful groups were relinquished on seventh day. Their correct kidney was inspected for the presence of calcium oxalate gems and stone development by histological procedures.
Result - The presence of crystals in the urine, elevated blood levels, and lowered urine levels of biochemical markers such urea, creatinine, sodium, potassium, and chlorides are signs of crystal deposition. The outcomes validated Mimosa pudica's beneficial impact on urolithiasis and supported the traditional Ayurvedic usage of this herb as an anti-urolithiatic medication.
Downloads
Article Details
This work is licensed under a Creative Commons Attribution 4.0 International License.
References
A.Mariappan*, G. (2016). Evaluation of Anti-Urolithiatic Potential of Novel Siddha Formulation Seenakaraparpam on Ethylene Glycol-Induced Urolithiasis in Wistar Albino Rats. Indian Journal of Medical Research and Pharmaceutical Sciences, 3(December), 23–31.
https://doi.org/10.5281/zenodo.197062
Alelign, T., Urology, B. P.-A. in, & 2018, U. (n.d.). Kidney stone disease: an update on current concepts. Hindawi.Com. Retrieved December 14, 2023, from
https://www.hindawi.com/journals/au/2018/3068365/abs/
Bhuvaneswari, P., & Krishnakumari, S. (2012). Nephroprotective effects of ethanolic extract of Sesamum indicum seeds (Linn.) in streptozotocin induced diabetic male albino rats. International Journal of Green Pharmacy, 6(4), 330–335. https://doi.org/10.4103/0973-8258.108249
Brckner, H., & Schieber, A. (2001). Determination of amino acid enantiomers in human urine and blood serum by gas chromatography-mass spectrometry. Biomedical Chromatography, 15(3), 166–172. https://doi.org/10.1002/bmc.57
Cimen, S., Eraslan, A., … A. M.-E. R. for, & 2023, undefined. (n.d.). Clinical characteristics and short-term outcomes of adult patients with urolithiasis: first report from Somalia. Europeanreview.Org. Retrieved December 14, 2023, from https://www.europeanreview.org/wp/wp-content/uploads/7127-7133.pdf
Das, S., Vasudeva, N., & Sharma, S. (2019). Kidney disorders and management through herbs: A Review. The Journal of Phytopharmacology, 8(1), 21–27. https://doi.org/10.31254/phyto.2019.8106
Eisner, B., Goldfarb, D., Clinics, G. P.-U., & 2013, undefined. (2013). Pharmacologic treatment of kidney stone disease. Urologic.Theclinics.Com, 40, 21–30. https://doi.org/10.1016/j.ucl.2012.09.013
Elmas, O., Erbas, O., & Yigitturk, G. (2016). The efficacy of Aesculus hippocastanum seeds on diabetic nephropathy in a streptozotocin-induced diabetic rat model. Biomedicine and Pharmacotherapy, 83, 392–396. https://doi.org/10.1016/j.biopha.2016.06.055
Eredics, K., Drerup, M., Özsoy, M., … C. W.-W. J. of, & 2023, undefined. (n.d.). Active stone removal is a safe option for ocotogenarians and nonagenarians with nephrolithiasis. Springer. Retrieved December 14, 2023, from https://link.springer.com/article/10.1007/s00345-023-04304-3
Feng, J., Wang, H., Jing, Z., Wang, Y., … W. W.-F. in, & 2021, undefined. (n.d.). of the Trace Elements Zinc and Magnesium With Diabetic Nephropathy-Associated Renal Functional Damage in Patients With Type 2 Diabetes Mellitus. Frontiersin.Org. Retrieved August 7, 2021, from
https://www.frontiersin.org/articles/10.3389/fmed.2021.626909/full
Ghule, A., Jadhav, S., urology, S. B.-A. journal of, & 2011, undefined. (2019). Renoprotective effect of Linum usitatissimum seeds through haemodynamic changes and conservation of antioxidant enzymes in renal ischaemia-reperfusion injury. Taylor & Francis, 9(3), 215–221.
https://doi.org/10.1016/j.aju.2011.07.007
Hashemi, E., … H. D.-J. of translational, & 2021, undefined. (n.d.). WT1 and ACE mRNAs of blood extracellular vesicle as biomarkers of diabetic nephropathy. Springer. Retrieved August 7, 2021, from https://link.springer.com/article/10.1186/s12967-021-02964-6
Jorg, T., Halfmann, M. C., Rölz, N., Mager, R., Pinto dos Santos, D., Düber, C., Mildenberger, P., & Müller, L. (2023). Structured reporting in radiology enables epidemiological analysis through data mining: urolithiasis as a use case. Abdominal Radiology, 48(11), 3520–3529. https://doi.org/10.1007/S00261-023-04006-9
Karakuş, A., Değer, Y., failure, S. Y.-R., & 2017, undefined. (2016). Protective effect of Silybum marianum and Taraxacum officinale extracts against oxidative kidney injuries induced by carbon tetrachloride in rats. Taylor & Francis, 39(1), 1–6. https://doi.org/10.1080/0886022X.2016.1244070
Lakshmi, T., & Devaraj, E. (2017). Antiurolithiatic activity of phytochemical extracts: A review. Journal of Advanced Pharmacy Education and Research, 7(3), 200–203.
Medicine, M. H.-J. of S., & 2023, undefined. (2023). Correlation of Kidney Volume on CT-Scan with Kidney Function Tests in Patients with Normal Kidneys at Haji Adam Malik Hospital Medan in 2020-2021. Jsocmed.Org, 2(4), 117–126. https://doi.org/10.47353/jsocmed.v2i4.46
Muhammad, G., Hussain, M. A., Jantan, I., & Bukhari, S. N. A. (2016). Mimosa pudica L., a High-Value Medicinal Plant as a Source of Bioactives for Pharmaceuticals. Comprehensive Reviews in Food Science and Food Safety, 15(2), 303–315. https://doi.org/10.1111/1541-4337.12184
Patel, R. K., Patel, S. B., & Shah, J. G. (2011). Anti-urolithiatic activity of ethanolic extract of seeds of Benincasa hispida (Thumb). Pharmacologyonline, 3, 586–591.
Paterson, R., Fernandez, A., … H. R.-C. U., & 2010, undefined. (n.d.). Evaluation and medical management of the kidney stone patient. Ncbi.Nlm.Nih.Gov. Retrieved December 15, 2023, from https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2997825/
Powell, M., Wheatley, A., Omoruyi, F., Asemota, H., Williams, N. P., & Tennant, P. F. (2008). Effects of subchronic exposure to transgenic papayas (Carica papaya L.) on liver and kidney enzymes and lipid parameters in rats. Journal of the Science of Food and Agriculture, 88(15), 2638–2647. https://doi.org/10.1002/JSFA.3383
Ramello, A., Vitale, C., nephrology, M. M.-J. of, & 2001, undefined. (n.d.). Epidemiology of nephrolithiasis. Academia.Edu. Retrieved December 14, 2023, from
https://www.academia.edu/download/43452111/Epidemiology_of_nephrolithiasis20160307-13252-tszdr8.pdf
Ravi, K., … B. R.-B. and, & 2004, undefined. (2004). Protective effect of Eugenia jambolana seed kernel on tissue antioxidants in streptozotocin-induced diabetic rats. Jstage.Jst.Go.Jp.
https://www.jstage.jst.go.jp/article/bpb/27/8/27_8_1212/_article/-char/ja/
Saleem, M., Asif, M., Parveen, A., Yaseen, H. S., Saadullah, M., Bashir, A., Asif, J., Arif, M., Khan, I. U., & Khan, R. U. (2021). Investigation of in vivo anti-inflammatory and anti-angiogenic attributes of coumarin-rich ethanolic extract of Melilotus indicus. Inflammopharmacology, 29(1), 281–293. https://doi.org/10.1007/S10787-020-00703-9
Şam, E., Ekşi, M., Akkaş, F., Baytekin, H. F., Güler, E. M., Şimşek, A., Atar, F. A., Koçyiğit, A., & Taşçı, A. İ. (2023). The Effects of Viburnum Opulus L. on Kidneys of Rats with Ethylene Glycol-induced Nephrolithiasis. Yeni Üroloji Dergisi, 18(3), 216–229. https://doi.org/10.33719/yud.2023-18-3-1345876
Sharma, V., Sharma, N., Sharma, S., & Sharma, M. (n.d.). In-vitro evaluation of antinephrolithiatic effect of selected medicinal plants (Bryophyllumpinnatum, Durantaerecta, Cynodondactylon, Duchesnea indica. Researchgate.Net. Retrieved December 14, 2023, from https://www.researchgate.net/profile/Shweta-Sharma-92/publication/375997292_In-vitro_evaluation_of_antinephrolithiatic_effect_of_selected_medicinal_plants_Bryophyllumpinnatum_Durantaerecta_Cynodondactylon_Duchesnea_indica_Taraxacum_officinalis/links/6566aca53fa26f66f4358c7a/In-vitro-evaluation-of-antinephrolithiatic-effect-of-selected-medicinal-plants-Bryophyllumpinnatum-Durantaerecta-Cynodondactylon-Duchesnea-indica-Taraxacum-officinalis.pdf
SINGH, P., SINGH, M., & MAHOR, A. (2023). “Urolithiasis (Kidney Stone) Treatment”: A Comprehensive Herbs-based Research Study.
Suvarchala Reddy, N. V. L., Ganga Raju, M., & Mamatha, M. (2021). Antilithiatic activity of leaf extract of citrus medica on sodium oxalate urolithiais-in-vitro and in-vivo evaluation. International Journal of Pharmaceutical Sciences and Research, 12(7), 3709–3715. https://doi.org/10.13040/IJPSR.0975-8232.12(7).3709-15
Times, J. T.-N., & 2023, undefined. (2023). Renal system 4: causes, diagnosis and treatment of chronic kidney disease. Emap-Moon-Prod.S3.Amazonaws …. http://emap-moon-prod.s3.amazonaws.com/wp-content/uploads/sites/3/2023/04/230424-Renal-system-4-causes-diagnosis-and-treatment-of-chronic-kidney-disease.pdf
Van Cangh, P., Smith, A. D., & Ng, C. S. (2007). Management of difficult kidney stones. Journal of Endourology, 21(5), 478–480. https://doi.org/10.1089/END.2007.9965
Yang, K., Zhang, L., Chen, W., Cheng, J., Zhao, X., & Zhang, Y. (2023). Expression of EPO and related factors in the liver and kidney of plain and Tibetan sheep. https://digitum.um.es/digitum/handle/10201/135785