Innovative eDNA Approaches For Fish Biomass Estimation In Aquatic Environments
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Abstract
We conducted research utilizing environmental DNA (eDNA) derived from aquatic vertebrates to assess species presence, with a specific focus on estimating biomass. Our hypothesis posited that fish release DNA into the water proportionate to their biomass, enabling the use of eDNA concentration to estimate species biomass. A novel eDNA method was developed for estimating the Rohu fish, Labeo rohita (Hamilton, 1822) biomass through both laboratory and field experimentation. In aquarium settings, we observed a dynamic change in eDNA concentration initially, stabilizing after 6 days. Notably, temperature exhibited no significant impact on eDNA concentrations in aquarium environments. Positive correlations between fish biomass and eDNA concentration were identified in both aquarium and experimental pond settings. Subsequently, we applied this eDNA method to estimate Rohu fish biomass and distribution in a natural freshwater ecosystem. Our findings indicated modifying the distribution of fish eDNA concentration could be revealed by water temperature. Consequently, we propose that biomass data derived after eDNA concentration serves as a reliable indicator of the likely distribution of carp in natural environments. The measurement of eDNA concentration offers a non-invasive, straightforward, and rapid approach to biomass estimation. This method holds promise for informing management plans geared toward ecosystem conservation.
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