Prevalence of Siderophore Genes in Faecal Escherichia Coli Isolates From Different Captive Animal Species
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Abstract
Faecal matter collected from 66 healthy wild captive animal species of two different sites of Rajasthan (Bikaner zoo and Machia biological park, Jodhpur) was examined for the presence of E. coli which carries a broad range of virulence associated genes responsible for intestinal and extraintestinal infections. The objectives of this study were to isolate and characterize E. coli from different wild captive animal species and to determine their siderophore gene profiles by PCR. A total of 66 faecal samples were analysed for the presence of E. coli and 59 isolates were positively identified as E. coli. 16S rRNA sequence data were used to confirm the identity of the isolates. PCR were performed to amplify fyuA and iroN genes fragment which are involved in iron acquisition and thus contribute to bacterial virulence. we reported high prevalence of fyuA (89.83%) and comparatively low prevalence of iroN (40.67%) in both Bikaner and Jodhpur regions.
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