Study of Antimicrobial Activity & Structural Elucidation of Purified Compound from Potential Solvent Extract of Bruguiera cylindrica [L]
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Abstract
B.Cylindrica is used as a mangrove plant in folk medicine to cure various diseases and disorders which include tumors, blood clotting, infections, fevers, pains etc., The Present study was to isolation, purification and characterization of antibacterial compound from the leaves along with phytochemical analysis. Healthy leaves of B. cylindrica were collected from natural habitat, washed, air dried and ground into powder. The solvents, such as chloroform, ethyl acetate, ethanol, methanol, and butanol were used to extract the powdered leaves. Crude extracts were tested for phytochemical constituents like carbohydrates, glycosides, proteins, steroids, triterpenoids flavonoids, alkaloids, tannins, saponins and phenols. Antimicrobial activities for crude extracts were tested against bacteria and fungi using standard well diffusion agar plate method. The highest antibacterial activity was identified in ethyl acetate extract, which was further purified using column chromatography. Activity-guided ethyl acetate fraction contains potential compound was used for structure elucidation using LC-MS, FTIR and NMR studies. Mass spectrometry of purified compound showed major peak at 6.901 minutes with a molecular weight of 256.32 g/mol, suggesting that the compound belongs to carboxylic acid group with a molecular formula CHN2O3 when compared to LC-MS standard from the NIST library. The Pure compound from Bruguiera cylindrica showed the stretch at 2561 indicated the presence of (O-H) hydroxyl group, 3341 N- H group, a stretch at 1709 indicated the presence of C=O group. The data obtained showed the presence of nitrogen containing amine, amide and carboxylic acid functional groups 7-(dimethylamino)-3ethyl-8-oxo-5-thia-1-azabicyclo(4.2.0)-oct-2-ene-2-carboxylic acid with a molecular formula CHN2O3. Based on 1H NMR spectrum and database of NIST, purified fraction was identified as 7-(dimethylamino)-3ethyl-8-oxo-5-thia-1-azabicyclo(4.2.0)-oct-2-ene-2-carboxylic acid. The results show that B cylindrica contains significant phytochemical and antibacterial activity against tested bacteria and fungi that helps to scientifically understand potentiality of tribal medicine.
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