Evaluation Of Ameliorative Effect Of Taurine And Metformin In High Fat Diet-Induced Diabetes
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Abstract
The aim of the study was to evaluate the anti-diabetic effect of Taurine (TAU), singly and in combination with Metformin (MET) High Fat Diet (HFD) induced diabetic rats.For this purpose, male Albino wistar male, 200–250 g in weight, assigned to groups of five (5). The rats were fed on either normal pellet diet (NPD) (4.1% fat, 22.2% protein, and 12.1% carbohydrates, as a percentage of total kcal) or high fat diet (HFD) (58% fat, 25% protein and 17% carbohydrate, as a percentage of total kcal) ad libitum, for an initial period of three weeks. Either MET, TAU or MET-TAU (each at 2.4 mM/kg, in water). Control rats received only distilled water and normal diet. After the study duration, the blood samples were withdrawn through retro-orbital plexus and blood glucose level (GLC) and insulin (INS) were measured. Further, various lipid parameters were also observed from blood. Immediately thereafter, the liver were surgically removed and a portion was used to prepare a homogenate in 0.1 M PBS pH 7.4, which was used for the for the determination of various parameters from the liver. Diabetes group raised the plasma GLC level (497.1±9.3 mg/dL in HFD) but lowered that of the blood INS (27.7±3.5 μU/mL) compared to corresponding values from nondiabetic rats (GLC =88.5±3.2 mg/dL and INS=43.7±4.8 μU/mL). In addition, it raised the liver malondialdehyde (MDA) level but lowered the reduced glutathione (GSH), and glutathione disulfide (GSSG) significantly (all at p < 0.01) was also observed in the normal diet and HFD rats. After the treatment, with TAU and MET alone or in combination, the significant reduction of GLC and enhancement of INS was observed. Moreover, MDA level in liver reduced but enhanced the GSH, and GSSG was also observed significantly (all at p < 0.01) in the normal diet and HFD rats after administration of TAU alone or in combination with MET. Finally, it is was concluded that the effect of TAU alone on various tissue and liver parameters is comparable to MET on both normal diet and HFD rats but their combination exhibited a synergistic effect on tissue, liver parameters, and on GLC as well as on INS.
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