The Effect Of Heavy Metal Cd On The Histology Of Male Nilem Fish (Osteochilus hasselti)

Main Article Content

Norman Arie Prayogo
Purnama Sukardi
Asrul Sahri Siregar
Yasumasa Bessho
Ren Fitriadi
Waheti Aulia Sarasih

Abstract

Cd is a heavy metal that is found in many aquatic environments and has toxic effects on aquatic organisms. Cadmium has a negative impact on organisms reproductive systems, especially during the process of spermatogenesis. The test biota used in this study was male nilem fish (Osteochilus hasselti). The purpose of this study was to determine the effect of heavy metal Cd on the level of damage to the testicular tissue of male nilem fish and to determine the ratio of the level of damage to the testicular tissue of male nilem fish in four treatments of Cd heavy metal for 4 weeks. The method used was an experimental method with a completely randomized design research design (CRD). The research was conducted in three stages, namely taking the testes, making histological preparations, and laboratory analysis using a microscope. The test biota was given four treatments of Cd 0 ppm, 2 ppm, 4 ppm, and 6 ppm with an exposure time of 28 days and sampling was carried out every two weeks. Quantitative data in the form of the proportions of the spermatogenesis stages were analyzed by One Way ANOVA. The results showed that there was no change in shape at the spermatogenesis stage and showed fluctuating results. The results showed that giving 4 Cd treatments to fish for 4 weeks did not affect spermatogenesis.

Downloads

Download data is not yet available.

Article Details

How to Cite
Norman Arie Prayogo, Purnama Sukardi, Asrul Sahri Siregar, Yasumasa Bessho, Ren Fitriadi, & Waheti Aulia Sarasih. (2024). The Effect Of Heavy Metal Cd On The Histology Of Male Nilem Fish (Osteochilus hasselti). Journal of Advanced Zoology, 45(1), 265–277. https://doi.org/10.53555/jaz.v45i1.3132
Section
Articles
Author Biographies

Norman Arie Prayogo

Program Study of Aquatic Resources Management and Magister SDA Program, Faculty of Fisheries and Marine Sciences, Universitas Jenderal Soedirman. Jl. Dr Soeparno, Banyumas 53122, Central Java, Indonesia

Purnama Sukardi

Departmen of Aquaculture, Faculty of Fisheries and Marine Science, Jenderal Soedirman University, Karangwangkal, Purwokerto 53122, Indonesia

Asrul Sahri Siregar

Program Study of Aquatic Resources Management and Magister SDA Program, Faculty of Fisheries and Marine Sciences, Universitas Jenderal Soedirman. Jl. Dr Soeparno, Banyumas 53122, Central Java, Indonesia

Yasumasa Bessho

Nara Institute of Science and Technology, Gene Regulation Research Laboratory, Division of Biological Science, Graduate School of Science and Technology, Takayama, Ikoma, Nara, Japan

Ren Fitriadi

Departmen of Aquaculture, Faculty of Fisheries and Marine Science, Jenderal Soedirman University, Karangwangkal, Purwokerto 53122, Indonesia

Waheti Aulia Sarasih

Program Study of Aquatic Resources Management and Magister SDA Program, Faculty of Fisheries and Marine Sciences, Universitas Jenderal Soedirman. Jl. Dr Soeparno, Banyumas 53122, Central Java, Indonesia

References

Almeida, J.A., Barreto, R.E., Novelli, L.B., Castro, F.J., Moron, S.E. 2009. Oxidative Stress Biomarkers and Aggressive Behavior in Fish Exposed to Aquatic Cadmium Contamination. Neotropical Ichtyology., 7: 103-108p. https://doi.org/10.1590/S1679-62252009000100013

Almeida-Santos, S. M., Braz, H. B., Santos, L. C., Sueiro, L. R., Barros, V. A., Rojas, C. A., & Kasperoviczus, K. N. 2014. Biologia reprodutiva de serpentes: recomendações para a coleta e análise de dados. Herpetologia Brasileira., 3(1): 14-24. https://www.researchgate.net/publication/261541731

Anggitasari, L., Suprapto, H., & Nindarwi, D. D. 2019. Change in Two-Spot Catfish Histopathological Liver (Mystus nigriceps) Accumulated with Heavy Metal Cadmium (Cd) in Ketingan Estuary, Sidoarjo-East Java, Indonesia. In IOP Conference Series: Earth and Environmental Science., 236(1): 012105. https://doi.org/10.1088/1755-1315/236/1/012105

Ardiyansyah, O., Sudarno., & Rosmanida. 2019. Bioaccumulation of cadmium (Cd) heavy metal on seaweed (Gracilaria sp.) in traditional fishpond of Jabon Subdistrict, Sidoarjo District. In IOP Conference Series: Earth and Environmental Science., 236(1): 012059. https://doi.org/10.1088/1755-1315/236/1/012059

Atli, G., Ariyurek, S. Y., Kanak, E. G., Canli, M. 2015. Alterations in the serum biomarkers belonging to different metabolicsystems of fish (Oreochromis niloticus) after Cd and Pb exposures. Environmental Toxicology and Pharmacology., 40(2): 508-515. https://doi.org/10.1016/j.etap.2015.08.001

Banks. W.J. 1986. Applied Veterinery Histology, 2nd ed.USA. The Williams and Wilkins Company.

Darmono, 2001. Environment and Pollution (Relationship with Metal Compound Toxicology). University of Indonesian Press. Jakarta.

Drąg-Kozak, E., Łuszczek-Trojnar, E., Socha, M., & Bojarski, B. 2021. Effects of melatonin on cadmium accumulation and haematological parameters in cadmium intoxicated Prussian carp (Carassius gibelio B.). Annals of Animal Science., 21(3): 899-923. https://doi.org/10.2478/aoas-2020-0105

Dutta, H. M .1996. Fish Morphologiy: Horizon of New Research. Science Publisher.

Effendie, M. I. 1979. Fisheries Biology Methods. Yayasan Dewi Sri. Bogor

Effendie, I. M. 2002. Fisheries Biology. Yayasan Pustaka Nusantara. Bogor. 163 p

Effendi, M. I. 2006. Fisheries Biology. Second printing. Yayasan Pustaka Nusatama. Yogyakarta.

Erlangga. 2007. Pollution Effects of Melting Kampau River in Riau Province on Baung Fish (Hemibagus meminrus). Thesis. IPB Postgraduate School. Bogor.

Eroschenko, V. P. 2013. Difiore’s Atlas of Histology with Functional Correlation, 12th Ed. EGC. Idaho, Moscow.

Fira, D., Wiradana, P. A., Ansori, A., Susilo, R. J. K., & Sabdoningrum, E. 2021. Ectoparasite inventorisation of nilem fish (Osteochilus hasselti) fingerlings cultured on ponds in Sukabumi, West Java, Indonesia. Iraqi Journal of Veterinary Sciences., 35(3): 605-609. https://doi.org/10.33899/ijvs.2020.127031.1440

Gage, M. J. G, Macfarlane C. P, Yeates S, Ward R. G, Searle JB, Parker G. A. 2004. Spermatozoal traits and sperm competition in Atlantic salmon: relative sperm velocity is the primary determinant of fertilization success. Current Biology., 14: 44–47. https://doi.org/10.1016/j.cub.2003.12.028

Guyton, A.C., Hall, J. E. 2001. Reproductive and Hormonal Functions of the Male (and Function of the Pineal Gland). In: Textbook of Medical Physiology. Philadelphia. WB. Saunders.

Gosling, E. 1992. The Muscle Mytilus: Ecology, physiology geneties and cultures. Development in Aquaculture Fisheries Science, Volume 25. Elsevier London, New York, Tokyo. p. 442-459.

Harsanto, J.B. 1997. Environmental Pollution Parameters and Criteria in the Basics of Environmental Pollution Control. Environmental impact Control Agency (BAPEDAL), PPLH UGM, Yogyakarta.

Hayati, B., Maleki, A., Najafi, F., Daraei, H., Gharibi, F., and McKay, G. (2017). Super High Removal Capacities of Heavy Metals (Pb2+ and Cu2+) Using CNT Dendrimer. J. Hazard. Mater., 336: 146–157. https://doi.org/10.1016/j.jhazmat.2017.02.059

Hayati, A., Pramudya, M., Soepriandono, H., Suhargo, L., & Rahmah, F. 2023. Supplementary Feed Potential on Histology and Immune Response of Tilapia (Oreochromis niloticus L.) Exposed to Microplastics. Sains Malaysiana., 52(6): 1607-1617. http://doi.org/10.17576/jsm-2023-5206-01

Hendri, A., & Baihaqi, B. 2015. Gonad Maturity Level of Male Kerling Fish, Tor Tambroides (Cyprinidae) Caught in the Jambak River Watershed, West Aceh Regency: Histological Approach. Jurnal Perikanan Tropis., 2(2): 111-137. https://doi.org/10.35308/jpt.v2i2.22

Heryani, L. G. S., Susari, N. N., Kardena, M. I., Laksmi, I. D. N. D. 2011. Paparan Formalin Menghambat Proses Spermatogenesis pada Mencit. Jurnal Veteriner., 12(3): 214-220. https://ojs.unud.ac.id/index.php/jvet/article/view/3518

Insivitawati, E., Mahasri, G., & Kusnoto. 2015. Haematology and histopatology of gills, intestine and brain koi fish (Cyprinus carpio koi) myxobolus koi orally infected. Jurnal Ilmiah Perikanan dan Kelautan., 7(2): 225-234. https://doi.org/10.20473/jipk.v7i2.11210

Isroni, W., & Maulida, N. 2022. Accumulation of heavy metals Pb and Hg in feather shells (Anadara antiquata) in Lekok Coastal Waters, Pasuruan Regency. In IOP Conference Series: Earth and Environmental Science., 1036(1): 012091. https://doi.org/10.1088/1755-1315/1036/1/012091

Mehmood, M. A., Qadri, H., Bhat, R. A., Rashid, A., Ganie, S. A., Dar, G. H., Shafiq, ur-Rehman. 2019. Heavy metal contamination in two commercial fish species of a trans-Himalayan freshwater ecosystem. Environ Monit Assess Environ., 191:104. https://doi.org/10.1007/s10661-019-7245-2

Mescheq, A. L. 2009. Junqueira's basic histology: text & atlas. EGC.

Mohiuddin, K. M., Ogawa, Y., Zakir, H. M., Otomo, K., Shikazono, N. 2011. Heavy metals contamination in the water and sediments of an urban river in a developing country. International Journal of Environmental Science and Technology., 8: 723–736. https://doi.org/10.1007/BF03326257

Nalbandov, A. V. 1990. Reproductive Physiology in Mammals and Poultry. University of Indonesia, Jakarta.

Nisak, K., Rahardja, B. S., & Masithah, E. D. 2013. Studi Perbandingan Kemampuan Nannochloropsis sp. dan Chlorella sp. Sebagai Agen Bioremediasi Terhadap Logam Berat Timbal (Pb). Jurnal Ilmiah Perikanan Dan Kelautan., 5(2): 175–180. https://doi.org/10.20473/jipk.v5i2.11405

Nugraha, A., & Hendra, H. 2017. Techniques for Making Feed, Parenting, and Spawning Nilem Fish (Osteochilus hasselti). Buletin Teknik Litkayasa Akuakultur., 15(2): 77-81. http://dx.doi.org/10.15578/blta.15.2.2017.77-81

Palar, H. 2004. Heavy Metal Pollution and Toxicology. PT. Rineka Cipta.

Peraturan Pemerintah Republik Indonesia. Nomor 82 Tahun 2001 tentang Pengelolaan Kualitas Air dan Pengendalian Pencemaran Air.

Prayogo, N.A., Hidayati, A., Siregar, A.S., Yunasfi. 2016. Lethal and Sublethal Toxicity Test of the Heavy Metal Mercury (Hg) on Nilem Fish (Osteochilus hasselti). Omni Akuatika., 12(1): 86-94. http://dx.doi.org/10.20884/1.oa.2016.12.1.68

Prayogo, N. A., Siregar, A., Wijaya, H., Sukardi, P., & Fitriadi, R. 2023. Pengaruh Logam Berat Kadmium (Cd) Terhadap Ekspresi GEN cGnRH-II Pada Ikan Nilem (Osteochilus hasselti CV) Jantan. Jurnal Akuakultur Sungai dan Danau., 8(2): 107-114. http://dx.doi.org/10.33087/akuakultur.v8i2.168

Prabowo, Rossi. 2005. Accumulation Cadmium of Bandeng Fish Flesh. Mediagro., 1(2): 58-74. https://dx.doi.org/10.31942/md.v1i2.910

Purbonegoro, T. 2017. Factors that Influence the Toxicity of Pollutants to Aquatic Organisms. Oseana., 42(2): 12-22. http://dx.doi.org/10.14203/oseana.2017.Vol.42No.2.43

Purwoko, Y., & Triwahyudi, Z.E., 2010. The Effect of Giving Eurycoma Longifolia Extract on the Diameter of the Seminiferous Tubules of Male Balb/C Mice Stressed by Electrical Shock Stressors. Media Medika Muda (M3)., (4): 45 – 50. http://eprints.undip.ac.id/22193/

Rahayu, N.I., Rosmaidar, Hanafiah, M, Karmil, T.F., Helmi, T.Z., Daud, R. (2017). Effect of lead (Pb) exposure on the growth rate of tilapia (Oreochromis nilloticus). Veterinary Student Scientific Journal., 1(4): 658-665. https://doi.org/10.21157/jim%20vet..v1i4.4757

Shah, S. L., and Altindag, A. 2005. Effects of Heavy Metal Accumulation on the 96-h LC_50 Values in Tench Tinca tinca L., 1758. Turkish Journal of Veterinary and Animal Sciences., 29(1): 139-144p. https://journals.tubitak.gov.tr/veterinary/vol29/iss1/23/

Siregar, A. S., and Prayogo, N. A., 2017. The disruptive effect of mercury chloride (HgCl) on gene expression of gonadotrophin hormones and testosterone level in male silver sharkminnow (Osteochilus hasseltii C.V.) (Teleostei: Cyprinidae). The European Zoological Journal., 84(1): 436-443p. https://doi.org/10.1080/24750263.2017.1352040

Soegianto, A., Irawan, B., & Hamami. 2012. Bioaccumulation of heavy metals in aquatic animals collected from coastal waters of Gresik, Indonesia. Coastal Environments: Focus on Asian Regions., 144-154. https://doi.org/10.1007/978-90-481-3002-3_10

Soemirat, J. 2005. Environmental Toxicology. Gadjah Mada University Press, Yogyakarta.

Sukmaningsih, A., Ermayanti, I. A. M., Wiratmini, N. I., & Sudatri, N. W. 2009. The Disturbance on Spermatogenesis After Administration Of Monosodium Glutamate On Mice (Mus musculus L.). Journal of Biology., 15(2): 49-52. https://www.researchgate.net/publication/277232982

Sumantadinata, K. 1983. Breeding Pet Fish in Indonesia. Hudaya Literature. Jakarta

Suwarsito, S., & Sarjanti, E. 2014. Spatial Analysis of Heavy Metal Pollution in Sediment and Water Biota at the Serayu River Estuary, Cilacap Regency. Geo Edukasi., 3(1): 30-37. https://jurnalnasional.ump.ac.id/index.php/GeoEdukasi/article/view/587

Tandjung, H. S. D. 1992. Effect of low levels of cadmium chloride on fish spermatogenesis. Biology., 159-167. https://onesearch.id/Record/IOS2744.17373

Tantri, A. F., Lamid, M., & Sugijanto, S. 2021. Application of Cockle (Anadara granosa) Shell Waste as an Adsorbent of Heavy Metal Cadmium (Cd), Copper (Cu), and Lead (Pb). Journal of Aquaculture and Fish Health., 11(1): 97–105. https://doi.org/10.20473/jafh.v11i1.26916

Taylor, and Francis. 2009. Methods in reproductive aquaculture marine and freshwater species. CRC Press: NewYork, Suite 300.

The World Bank. 1983. Fish to 2030: prospects for fisheries and aquaculture.

Soeprijanto, H. 2003. Absorption of Heavy Metals Mercury and Cadmium in Tilapia mossambica Peters. Purification Journal., 4(3): 139-144. https://doi.org/10.12962/j25983806.v4.i3.337

Zikic, R.V., Stajn, A.S., Pavlovic, S.Z., Ognjanovic, B.I., Saicic, Z.S. 2001. Activities of Superoxide Dismutase and Catalase in Erythrocytes an Plasma Transaminases of Goldfish (Carassius auratus gibelio Bloch.) Exposed to Cadmium. Physiol. Res., 50: 105-111. http://www.biomed.cas.cz/physiolres

Zulkarnain, M. N. F., Rahardja, B. S., & Alamsjah, M. A. 2013. Studi kandungan logam berat kadmium (Cd) pada spesies ikan kembung (Rastrelliger kanagurta) dan kerang darah (Anadara granosa) di Perairan Manyar, Gresik dan di Perairan Jabon, Sidoarjo. Jurnal Ilmiah Perikanan Dan Kelautan., 5(1): 37–42. https://doi.org/10.20473/jipk.v5i1.11422