Characterization Studies of Medicinal Plants & I’ts Biological Evaluation Towards Anti-Bacterial Study

Abstract

The traditional system of medicine proves to be one of the best sources for treating illness and infections. Murraya koenigii (curry leaves) and Asparagus racemosus (shatavari) are the two medicinal plant species that have numerous medicinal properties like antimicrobial, anticancer, anti-inflammatory, etc. In this study, the Ultrasound extraction and the Soxhlet mode of extraction were carried out using two different solvents such as Hexane and Ethyl acetate. Analysis and Retention factor were was carried out thin layer chromatography. The purification was performed using column chromatographic method and the various phytoconstituents such as tannins, terpenoids, carbohydrates, proteins, steroids, flavonoids, phlobatannins were analysed for the extracted samples. Retention factors have been calculated for both the extracted samples and the samples were further processed for sterility test in order to check for any contamination. The extracted samples were characterized using GC-MS analysis. The anti-bacterial evaluation was performed. The highest zone of inhibition found in 1000µg/ml concentration for Pseudomonas aeruginosa and 500µg/ml concentration for Staphylococcus aureus. The minimum inhibitory concentration for Pseudomonas aeruginosa is predicted as 250µg/ml concentration and for Staphylococcus aureus as 500µg/ml concentration. The rate of kill was higher in 500µg/ml concentration for both bacterial samples. The crystal violet assay was performed and observed the cell viability. Further the In-silico analysis was performed towards the GC analysis compounds and their structure activity relationship were studied towards the protein.