MORPHOFUNCTIONAL CHARACTERISTICS OF CELLULAR ELEMENTS OF RAT TESTES UNDER THE INFLUENCE OF INSECTICIDE ROVIKURT

Abstract

The purpose of the study was to study the characteristics of the response of germ and somatic cells to the action of rovicurt, as well as to study some aspects of the mechanism of this action.

The research materials were the new insecticide Rovicurt, produced by the company “Hinoin” (Hungary) in the form of a 20% emulsion solution, proposed as a systemic acaricide. It mixes well with most organic solvents and consists of 23% Ambush (permethrin) and 2% tetramethrin (C₂₁H₂₀Cl₂O₃+C₁₇H₂₅O₄).

The experiment used white male and female rats weighing 150-200 g. To study this process, male rats (132 pcs.) were taken, subjected to a single action of rovicurt in doses of 380, 150, 75 mg/kg, as well as control animals. The studies were carried out on days 1, 3, 7, 14, 21 and 70 after exposure. And with repeated administration - doses of 38, 19 mg/kg for 2.5 months, as well as control animals.

Research methods. Samples of testicular tissue were fixed in Carnoy's fluid and 2.5% glutaraldehyde solution, followed by additional fixation with 1% osmium tetroxide solution, dehydrated in alcohols of increasing strength, embedded in paraffin and in a mixture of epons. Paraffin sections were stained with hematoxylin-eosin, semi-thin sections prepared on an LKB-4800 ultratome were stained with methylene blue.

Results. When exposed to rovicurt at a dose of 380 mg/kg, the proliferation of testicular stem cells (spermatogonia) is disrupted. This process is based on degenerative changes. Different populations of germ cells react differently to a single action of rovicurt (at doses of 75 and 159 mg/kg): differentiation of early and middle spermatids is suppressed, while late spermatids are activated. When exposed to pesticides in doses of 75, 150 and 380 mg/kg, damage to the genome of spermatogenic epithelial cells is observed.

Conclusion. With acute and chronic action of pyrethroids, a change in the fine structure of the synaptonemal complex occurs, a delay in the differentiation of spermatocytes and spermatids, the appearance of multinucleated cells, as well as structural changes in sustentocytes and specialized connections between neighboring sustentocytes and germ cells.