Extraction and Identification of Glycerides in Sesamum indicum Oil Seeds from Manipur

Abstract

Seed oil from Sesamum indicum was obtained by solvent extraction technique where petroleum ether was used as the extracting solvent after crushing the kernels. The oil was purified before going to further analysis achieved through column chromatography using silica gel (60-120 mesh) and an eluent mixture of petroleum ether and ethyl acetate (20:1). Subsequently, transesterification is done on the purified oil to produce biodiesel, also known as fatty acid methyl ester (FAME). The FAME composition of Sesamum indicum oil was determined using NMR, IR, GCMS analysis. The FAME profile of Sesamum indicum oil consists of 15.35 wt. % methyl palmitate [C16:0], 67.03 wt. % of methyl linoleate [C18:2], 14.92 wt. % of methyl stearate [C18:0] and 2.70 wt. % of methyl arachidate [C20:0].