Evaluation Of Alocasia Cordfolia Rhizomes Extract for Its Potential To Inhibit Oligomeric Amyloid β‑Induced Microglial Activation Through NADPH Oxidase

Abstract

Objective: The search for natural neuroprotective components from plants has received extensive attention worldwide. The purpose of this study was to examine the inhibitory effect of Alocasia cordfolia rhizomes extract (ACRE) on Oligomeric Amyloid β (oAβ)-induced microglial activation, and further to assess whether this effect is mediated through NADPH oxidase. Methods: Microglia activation model was established by inducing BV-2 microglia cells with oAβ. After treated with the extract for 24 h, cell viability was detected using cell counting kit-8 (CCK-8) assay, intracellular levels of ROS, MDA and SOD, as well as contents of NO, IL-1β and TNF-α in cell culture media were analyzed with commercial kits. Next, the protein expression of gp91phox was determined by Western blot. Results: In oAβ-stimulated BV-2 microglia cells, cell proliferation was significantly increased, intracellular ROS and MDA levels were markedly raised while SOD activity was reduced, and contents of NO, IL-1β and TNF-α in cell culture media were distinctly elevated. Meanwhile, gp91phox protein expression was obviously increased. However, these effects could be dramatically reversed dose-dependently by ACRE. Conclusion: ACRE may be a potentially promising natural neuroprotective products for the treatment of AD, due to its excellent activity in inhibiting microglial proliferation and activation induced by oAβ.