A Study on Effect of Microbial Growth on Dentures in Relation to Denture Storage and Overnight Soaking of Dentures in Various Soaking Solutions -An In Vitro Study

Abstract

Background: Denture hygiene is essential for preventing microbial growth and associated oral health issues in denture wearers. This in vitro study investigates the effect of denture storage conditions and overnight soaking in various solutions on microbial growth on denture surfaces. Methods: Standardized denture models were inoculated with Candida albicans, Streptococcus mutans, and Staphylococcus aureus and subjected to dry and moist storage conditions. Soaking solutions included commercial denture cleanser, sodium hypochlorite, hydrogen peroxide, and distilled water (control). Microbial assessments were conducted at specified intervals. Results: Moist storage significantly promoted microbial growth on dentures compared to dry storage. Soaking solutions, especially commercial denture cleanser, sodium hypochlorite, and hydrogen peroxide, demonstrated substantial reductions in microbial counts compared to distilled water. Conclusion: Denture storage conditions and choice of soaking solution significantly influence microbial growth on dentures. Moist storage and the use of antimicrobial solutions are effective strategies for reducing microbial colonization. Keywords: Denture hygiene, microbial growth, denture storage, soaking solutions, oral health.