Acute Exposure to Glufosinate Ammonium Induces Tissue Glycogen Depletion in the Freshwater Fish Labeo rohita
Keywords:
Labeo rohita, Glufosinate Ammonium, Sweep Power, Glycogen, Herbicide, Biochemical Biomarker, Ecotoxicology.Abstract
Herbicides are extensively used in agriculture and frequently contaminate aquatic ecosystems through surface runoff, posing a potential risk to non-target aquatic organisms. The present study evaluated the effect of acute exposure to Sweep Power (Glufosinate Ammonium) on the total glycogen content in different tissues of the freshwater fish Labeo rohita. Healthy fingerlings were exposed to LC₀ (0.01 ppm) and LC₅₀ (0.05 ppm) concentrations of the herbicide for 96 hours. Total glycogen content in the liver, muscle, brain, and gill tissues was estimated by the method of De Zwaan and Zandee (1972). A significant (P < 0.001) reduction in glycogen content was observed in all tissues of the treated groups compared to the control, with a greater decline at the LC₅₀ concentration. The depletion of glycogen indicates enhanced glycogenolysis and increased energy demand under herbicide-induced stress. The present findings suggest that tissue glycogen is a sensitive biochemical biomarker for assessing the toxic effects of Glufosinate Ammonium in freshwater fishes.
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