Biochemical Study and Molecular Approaching on Eight Wild Herbals Used in Folk Medicine in Al-Baha Region, KSA

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Aldhebiani A.Y.
Salah E. M. Abo-Aba
Alshehri M.H.
Hussein R.M.

Abstract

studies   describing   the   traditional   therapeutic   uses   of   natural   medicinal   and   aromatic   plants   have been established   in   Al-Baha   region, KSA.   In   this work, the antioxidant   activity   of   eight   herbals   plant   samples   from   Al-Baha   region   was   studied   using   different   solvents (ethanol, hexane   and water) to   evaluate   their   strong   and   total   lipid-soluble   antioxidant   and   water-soluble   antioxidant   activity (TLAC37,95 and TWAC37,95). The eight herbal plants were also molecularly   characterized   using   the   PCR-based   ISSR   markers. The studied samples demonstrated   valuable   and   variable   antioxidant   properties.   The   strong   antioxidant   capacity   of   Rumex   (Rumex   nervosus Vahl.)   samples   for   ethanolic   and   aqueous   extract   samples   was   the   highest   followed by Fringed Rue (Ruta chalepensis L.) samples (47.68 and 42.72 & 81.74 and 74.18   µmoles/g   respectively),   while   hexanic   extracts   of   Euryops   (Euryops   arabicus Steud. ex Jaub. & Spach) samples   revealed   the   highest   strong   antioxidant   capacity   followed   by   Fringed   Rue   samples   (23.67   and   13.83   µmoles/g   respectively).   Besides   that, ethanolic   extracts   of   Fringed   Rue   samples   demonstrated   the   highest   total   antioxidant   capacity   followed   by   olive   leaves (Olea europaea subsp. cuspidata (Wall. & G.Don) Cif.) samples (686.95 and 462.62 µmoles/g respectively), while aqueous   extracts   of   Rumex   samples   revealed   superior   total   antioxidant   capacity   followed   by   olive   leaves   samples   and   Fringed Rue samples (869.10, 837.21   and   743.75   µmoles/g   respectively.   But   the   hexanic   extracts   of   Fringed   Rue samples   revealed   valuable   total   antioxidant   capacity   followed by Rumex samples (127.83 and 38.00 µmoles/g respectively.   The   herbal   plants   of   Rumex, Fringed   Rue   and   olive revealed   huge   both   total   and   strong   antioxidant   activity.   In   the   present   study we began   an   initial   approach   to   authenticate   and   identify   different   plant species   in   our   study   using   inter-simple   sequence   repeats (ISSRs) molecular markers.

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How to Cite
Aldhebiani A.Y., Salah E. M. Abo-Aba, Alshehri M.H., & Hussein R.M. (2023). Biochemical Study and Molecular Approaching on Eight Wild Herbals Used in Folk Medicine in Al-Baha Region, KSA. Journal of Advanced Zoology, 44(S5), 2583–2595. https://doi.org/10.17762/jaz.v44iS5.2011
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