Molecular Characterization and Genetic Diversity Assessment of Lissemys punctata andersoni from Dhubri district of Assam, India
DOI:
https://doi.org/10.53555/jaz.v38i01.5305Keywords:
Genetic diversity, Molecular characterization, cytochrome-b, mitochondrial DNA, ConservationAbstract
The present study investigates the molecular characteristics and genetic diversity of Lissemys punctata andersoni across six populations in the Dhubri district of Assam using cytochrome b gene sequences. Base composition analysis revealed consistently AT-rich gene regions dominated by adenine, a common characteristic of mitochondrial DNA. Sequence alignment indicated both conserved and variable nucleotide regions across populations. In silico restriction site analysis using multiple enzymes demonstrated significant polymorphism, confirming genetic divergence among populations. Genetic clustering showed three distinct clades, suggesting population structuring and evolutionary divergence. The findings highlight the utility of cytochrome b as a molecular marker for genetic variability assessment and emphasize the importance of such studies for conservation and species management.
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Copyright (c) 2022 Bhabesh Nath, Chandralekha Deka
This work is licensed under a Creative Commons Attribution 4.0 International License.
