in vitro Effects of Nicotine on Lipid Peroxidation and Motility in Cattle Bull Ejaculated Spermatozoa
Main Article Content
Abstract
Tobacco smoking, driven mainly by nicotine consumption, is a known environmental factor adversely affecting male reproductive health. This study investigates the in vitro impact of nicotine on lipid peroxidation and motility in cattle bull ejaculated spermatozoa. Nicotine exposure induced a dose-dependent increase in lipid peroxidation, as evidenced by elevated malondialdehyde (MDA) levels measured over a 120-minute period. Lipid peroxidation was measured by thiobarbituric acid-reactive substances (TBARS) and was shown to escalate significantly in nicotine-treated samples. Concurrently, sperm motility decreased significantly in nicotine-treated groups compared to controls, suggesting compromised sperm function. The findings highlight oxidative stress, mediated by reactive oxygen species, as the principal mechanism underlying nicotine-induced sperm damage. This research underscores the detrimental effects of nicotine on sperm quality through increased oxidative membrane damage and reduced motility, emphasizing the importance of targeting oxidative stress to preserve male fertility in cattle bulls.
Downloads
Article Details
This work is licensed under a Creative Commons Attribution 4.0 International License.
References
• Agarwal, A., Prabhakaran, S., & Storey, B. T. (2003). Sperm oxidative stress and male infertility. Journal of Andrology, 24(5), 706–715.
• Agarwal, A., Saleh, R. A., & Bedaiwy, M. A. (2003). The Role of Reactive Oxygen Species in the Pathophysiology of Human Reproduction. Fertility and Sterility, 79(4), 829–843.
• Agarwal, A., Nallella, K. P., Allamaneni, S. S., & Said, T. M. (2004). Role of antioxidants in treatment of male infertility: an overview of the literature. Reproductive biomedicine online, 8(6), 616–627.
• Alvarez, J. G., & Storey, B. T. (1992). Role of glutathione peroxidase in protecting mammalian spermatozoa from loss of motility caused by spontaneous lipid peroxidation. Gamete Research, 32(3), 219–228.
• Benoff, S., Cooper, G. A., & Hurley, I. R. (2000). The role of oxidative stress in male infertility. Human Reproduction Update, 6(2), 105–114.
• Evans, J. L., & Kuhn, D. M. (1981). Mechanisms of nicotine-induced oxidative stress and apoptosis in cultured neuronal cells. Brain Research, 1099(1), 35–45.
• Guraya, S. S. (1987). Calcium regulation in spermatozoa: Relevance to fertility. Journal of Reproduction and Fertility, 79(2), 395–403.
• Ipsen, J., & Feigel, P. (Eds.). (1970). Bancroft’s introduction to biostatistics (2nd ed.). Harper and Row Publishers.
• Maridomen, I., Braquet, P., & Garay, R. P. (1979). Method of malonyldialdehyde measurement. Journal of Biological Chemistry, 5, 3107–3117.
• Mukai, R., Yamanouchi, K., Nakanishi, Y., & Saito, H. (2004). Nicotine-induced oxidative stress in male reproductive cells. Journal of Toxicology and Environmental Health, 67(9), 891–901.
• Rodriguez, I., Murphy, M. B., Flaherty, S., & Blasco, L. (1982). The effects of smoking on human sperm motility. Archives of Andrology, 8(3), 225–228.
• Saleh, R. A., Agarwal, A., Sharma, R. K., Nelson, D. R., & Thomas, A. J. (2003). Effect of cigarette smoking on levels of seminal oxidative stress in infertile men: A prospective study. Fertility and Sterility, 80(4), 771–777.
• Stillman, R. J., Grodstein, F., & Schottenfeld, D. (1986). Smoking and male infertility. Fertility and Sterility, 46(6), 1062–1066.
• Viczian, O. (1969). Effect of cigarette smoking on human spermatozoa. British Journal of Urology, 41(2), 173–174.
• World Health Organization. (2010). *Global health observatory*. Geneva: World Health Organization. Retrieved February 4, 2010, from https://www.who.int/data/gho
• Yanagimachi, R. (1975). Sperm capacitation and the acrosome reaction. Biology of Reproduction, 12(4), 592–604.
• Zini, A., Boman, J. M., Belzile, E., & Ciampi, A. (1999). Sperm DNA damage is associated with cigarette smoking. Fertility and Sterility, 71(4), 613–617.