Amino Acid Distribution Analysis for A Model Protein Bovine Serum Albumin (BSA)
DOI:
https://doi.org/10.53555/jaz.v37i01.4986Keywords:
Bovine Serum Albumin (BSA), Amino acid composition, GroupWise distribution of amino acidsAbstract
Serum albumin, a soluble protein found in blood plasma, interacts with various drugs, including anticancer drugs, antibiotics, and antiviral medicines. BSA, a bio-tool, has been extensively studied for interactions between bio-compounds and proteins. Its medicinal importance, stability, neutrality, accessibility, cost-effectiveness, and abundance make it a popular choice for drug delivery research. BSA is also essential for the manufacturing of viral vaccines, which require a cell culture medium. This thesis investigates the interactions between BSA and drugs under physiologically simulated settings due to their medical importance and unusual ligand-binding capabilities. BSA is more stable in a wider pH range and at higher temperatures, making it a viable candidate for protein-based drug delivery systems. This study analyses amino acid composition and group-wise distribution of BSA.
Downloads
References
Chang, Y., Zajicek, J., & Castellino, F. J. (1997). Role of tryptophan-63 of the kringle 2 domain of tissue-type plasminogen activator in its thermal stability, folding, and ligand binding properties. Biochemistry, 36(25), 7652–7663. https://doi.org/10.1021/bi970197g
Cox Michael M., D. L. N. (2015). Lehninger Principles of Biochemistry (6th ed.). W. H. Freemanand CoLLtd.
González, E., Álvarez, O., Díaz, Y., Parra, C., & Bustacara, C. (2005). BSA: A complete coverage algorithm. Proceedings - IEEE International Conference on Robotics and Automation, 2005(April), 2040–2044. https://doi.org/10.1109/ROBOT.2005.1570413
Hood, S. D., Bell, C. J., & Nutt, D. J. (2005). Acute Tryptophan Depletion. Part I: Rationale and Methodology. Australian & New Zealand Journal of Psychiatry, 39(7), 558–564. https://doi.org/10.1080/j.1440-1614.2005.01627.x
Lee, J., Park, I. S., Kim, H., Woo, J. S., Choi, B. S., & Min, D. H. (2015). BSA as additive: A simple strategy for practical applications of PNA in bioanalysis. Biosensors and Bioelectronics, 69, 167–173. https://doi.org/10.1016/j.bios.2015.02.030
Loughney, J. W., Lancaster, C., Ha, S., & Rustandi, R. R. (2014). Residual bovine serum albumin (BSA) quantitation in vaccines using automated Capillary Western technology. Analytical Biochemistry, 461(May), 49–56. https://doi.org/10.1016/j.ab.2014.05.004
Vogi, R. (1977). Bsa Sequence. 23(7), 1976–1977.
Weijers, R. N. M., & Peters, T. (1977). Amino acid sequence in bovine serum albumin. Clinical Chemistry, 23(7), 1361–1362. https://doi.org/10.1093/clinchem/23.7.1361
Downloads
Published
Issue
Section
License
Copyright (c) 2022 Vidya S. Patil, Pratibha S. Patil
This work is licensed under a Creative Commons Attribution 4.0 International License.
