Analysis Of The Anti-Oxidant And Cell Cytotoxicity Properties Of Stevia Rebaudiana Extract By Using In- Vitro Model
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Abstract
Leaves of Stevia rebaudiana are rich source of sweet glycosides of steviol. The major glycoside, stevioside, diterpenoid glycoside--is used in India and various countries as a food sweetener for diabetic patients. Its medical use is also reported in various ailments like heart, liver, pancrease etc. Besides this it is also being used against obesity, stomach burn and to lower uric acid levels in blood. In present study the leaf extract with various extract were tested for immunomodulatry properties. We performed MTT assay to check the cell viability and cytotoxicity in peripheral blood mononuclear cell (PBMC) and also calculated the Trolox equivalent values to assess the antioxidant properties of Stevia rebaudiana. . Percent cell viability values of cells were found to be more than 50 percentages with increase in concentration. At higher dose all these extract showed significant inhibitory effects on proliferation. The aqueous extracts shown the maximum value of Trolox equivalent with 1:16 dilution. It means with the increase in the concentration of extract there is substantial increase in antioxidant property or we can assume antioxidant is dose dependent. But in same time cell viability gets reduced with increase in concentration.
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