Optimizing Culture Medium And Growth Conditions To Enhance The Biomass And Quality Of Medical Mushroom Cordyceps Militaris Under Controlled Conditions

Abstract

Cordyceps militaris is widely used in many Asian countries as a traditional medicinal material for treating various ailments. This study aims to investigate how the growth of the medicinal mushroom C. militaris is influenced by the culture medium and culture conditions in a controlled laboratory setting. The results indicate that aromatic brown rice (BT7) significantly increases the fresh weight of all three collected materials of C. militaris. Shoot length and body weight were promoted by the glucose content, silkworm pupa powder and pH of the culture medium ranging from 6.0 to 7.0, with the optimal glucose content being 30.0g per bottle. The optimal temperature for growth is between 22°C and below 25°C, with a light intensity of 600-700 lux. For higher cordycepin content, the recommended harvesting time is 65 days, while for adenosine content should be 55 days after inoculation. These findings provide valuable information to improve both the biomass and quality of C. militaris, which is crucial for large-scale production in the pharmaceutical industry.