Isolation of Endophytic Bacteria from Palaquium Maingayi and their Ability to Promote Plant Growth

Authors

  • Muhammad Harith Iskandar Pakasa
  • Farah Eziana Sharaman
  • Wong Sie Chuong
  • Fauziah Abu Bakar

DOI:

https://doi.org/10.53555/jaz.v44iS7.4024

Keywords:

Fusarium solani, Ganoderma borneensis, Ralstonia solanacearum, maize, 16S rRNA, tricalcium phosphate

Abstract

Mutual interaction between endophytic bacteria and their plant host benefits both sides in a sustainable environment. To date, various endophytic bacteria with plant growth-promoting potential have been reported from a wide variety of plants. The present study aims to isolate endophytic bacteria with plant growth-promoting characteristics and potentially become biofertilizers to promote plant growth. These endophytic bacteria were isolated from the roots of Palaquium maingayi inhabiting Universiti Putra Malaysia Campus Bintulu (UPMKB) Borneo Base Forest. In total, 66 endophytic bacteria were isolated and examined for their phosphate solubilization, indole-3-acetic acid (IAA) production, siderophore production and antagonistic characteristics against pathogenic fungi and bacteria. Out of 66 endophytic bacterial isolates, 11 were able to solubilize phosphate, produce IAA and siderophore, and promote plant growth. 6 endophytic bacteria isolates were found to have antagonistic activities against Ganoderma borneensis, Fusarium solani and Ralstonia solanacearum. The results of plant inoculation showed that these bacteria strains significantly promote root and shoot biomass on maize plants compared to uninoculated plants. To our knowledge, this is the first report on isolating endophytic bacterial strains from the Palaquium Maingayi tree with plant growth-promoting potential. The success of isolation and identification of endophytic bacteria from selected trees in the UPMKB Borneo base forest can potentially produce bio-fertilizers to promote plant growth for sustainable agriculture.

 

Downloads

Download data is not yet available.

Author Biographies

Muhammad Harith Iskandar Pakasa

Department of Crop Science, Faculty of Agricultural and Forestry Sciences, Universiti Putra Malaysia Bintulu Sarawak Campus, Bintulu, Sarawak, Malaysia

Farah Eziana Sharaman

Department of Crop Science, Faculty of Agricultural and Forestry Sciences, Universiti Putra Malaysia Bintulu Sarawak Campus, Bintulu, Sarawak, Malaysia

Wong Sie Chuong

Department of Science and Technology, Faculty of Humanities, Management and Science,
Universiti Putra Malaysia Bintulu Sarawak Campus, Bintulu, Sarawak, Malaysia

Fauziah Abu Bakar

Institute of Ecosystem Science Borneo, University Putra Malaysia Bintulu Sarawak Campus, Bintulu

References

Abdul‐Baki, A. A., & Anderson, J. D. (1973). Vigor determination in soybean seed by multiple criteria 1. Crop science, 13(6), 630-633.

Abu Bakar, F., Iskandar Pakasa, M. H., Sharaman, F. E., Wong, S. C. & Lepun, P. Exploration of Plant Growth-Promoting Endophytic Bacteria from the Roots of Native Plant Uncaria Borneensis in Sarawak, Borneo. European Chemical Bulletin, 12(4), 12545-12554.

Afzal, I., Shinwari, Z. K., Sikandar, S., & Shahzad, S. (2019). Plant beneficial endophytic bacteria: Mechanisms, diversity, host range and genetic determinants. Microbiological research, 221, 36-49.

AHMAD, K. I., MAULANA, M., MAGIMAN, Z. R., SEEMAB, A., & Faridah-hanum, I. (2023). Diversity and utilization of ethnomedicinal plants in Sarawak, Borneo. The Malaysian Forester, 86(1), 125-152.

Brady, C. L., Cleenwerck, I., Van der Westhuizen, L., Venter, S. N., Coutinho, T. A., & De Vos, P. (2012). Pantoea rodasii sp. nov., Pantoea rwandensis sp. nov. and Pantoea wallisii sp. nov., isolated from Eucalyptus. International journal of systematic and evolutionary microbiology, 62(Pt_7), 1457-1464.

Gordon, S. A., & Weber, R. P. (1951). Colorimetric estimation of indoleacetic acid. Plant physiology, 26(1), 192.

Jasim, B., Jimtha, C. J., Jyothis, M., & Radhakrishnan, E. K. (2013). Plant growth promoting potential of endophytic bacteria isolated from Piper nigrum. Plant growth regulation, 71(1), 1-11.

Kumar, V., Jain, L., Jain, S. K., Chaturvedi, S., & Kaushal, P. (2020). Bacterial endophytes of rice (Oryza sativa L.) and their potential for plant growth promotion and antagonistic activities. South African Journal of Botany, 134, 50-63.

Pingale, S. S., & Virkar, P. S. (2013). Study of influence of phosphate dissolving microorganisms on yield and phosphate uptake by crops. Euro J Exp Biol, 3(2), 191-193.

Santoyo, G., Moreno-Hagelsieb, G., del Carmen Orozco-Mosqueda, M., & Glick, B.R. (2016). Plant growth-promoting bacterial endophytes. Microbiological research, 183, 92-99.

Schwyn, B., & Neilands, J. B. (1987). Universal chemical assay for the detection and determination of siderophores. Analytical biochemistry, 160(1), 47-56

Senthilkumar, M., Anandham, R., Madhaiyan, M., Venkateswaran, V., & Sa, T. (2011). Endophytic bacteria: perspectives and applications in agricultural crop production. In Bacteria in agrobiology: crop ecosystems (pp. 61-96). Springer, Berlin, Heidelberg.

Downloads

Published

2023-12-24

Issue

Section

Articles

Similar Articles

1 2 3 4 5 6 > >> 

You may also start an advanced similarity search for this article.