Synergistic Hepatoprotective Effect of Methanolic Extracts of Urtica Dioica LINN. Leaves and Silymarin CCL4 - Induced Hepatic Damage in HEPG2 Cells

Abstract

In this current study, the methanolic leaves extract of Urtica dioica Linn. (UDLE-M) was tested for its hepatoprotective and antioxidant properties. The extract's hepatoprotective activity was assessed in HepG2 cell lines against CCl4-causedhepatic injury. Additionally, in order to assess the antioxidant activityof UDLE-M in vitro, the assessment of total antioxidant activity was looked at.The synergistic effect of UDLE-M + Silymarin was also investigated in HepG2 cell lines. The extract exhibited noteworthy antioxidant activity, matching that of common antioxidant chemicals such as BHA (Butylated hydroxyanisole) and α-tocopherol, according to the data. A notable hepatoprotective profile was also noted, with an enhanced level of % cell viability suggesting that the dose of 110 µg/mL would result in the highest hepatoprotection. A significant synergistic hepatoprotective effect had also been observed for UDLE-M + Silymarin.