Qbd Approach Of Analytical Method Development And Validation Of Camylofin And Mefenamic Acid In Bulk And Pharmaceutical Dosage Form

Abstract

A simple, Accurate, precise method was developed for the estimation of the Camylofin and Mefenamic acid in bulk and pharmaceutical dosage form. The chromatogram was run through HSS 100 x 2.1 mm, 1.8. Mobile phase containing 0.1N Phosphate buffer: Methanol taken in the ratio 55:45 (% v/v) was pumped through the column at a flow rate of 0.2 ml/min. Temperature was maintained at 30°C. The optimized wavelength selected was ACQUITY TUV detector ChA 220.0 nm. Retention time of Camylofin was found to be 1.455 min Mefenamic acid was found to be 1.099 min. %RSD of the Camylofin and Mefenamic acid was found to be 0.9 and 0.9. %Recovery was obtained as 99.97% for Camylofin and 100.44 for Mefenamic acid. LOD and LOQ values obtained from regression equations of Camylofin were 0.03 and 0.08 respectively, and the Regression equation is y = 37722x + 1092.5. LOD and LOQ values obtained from regression equations of Mefenamic acid were 0.08 and 0.23 respectively, and the Regression equation is y = 30948x +6750.5. The chromatographic run time was decreased and method was rugged and robust, so the method developed was simple and economical and can be adopted in regular Quality control test in Industries.