Analysis Of Phytochemicals, Protein And Anti-Microbial Activity From The Latex Of Calotropis Procera
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Abstract
Calotropis procera is a perennial shrub with soft wood that is evergreen. It is a member of the Apocynaceae family and Asclepiadoideae subfamily. GC- MS (Gas chromatography-Mass Spectroscopy) is used to detect compounds. On performing GC-MS, 52 peaks for various substances are produced. The quantitative analysis of Methanolic, Ethanolic, and Crude extracts of Calotropis procera is done for secondary metabolites such as flavonoids and phenols, is carried out by using Gallic acid and Rutin as a standard. Various methods are used for the qualitative test for Alkaloids, Flavonoids, Phenols, Tannins, Saponins, Cardiac glycosides, Resins, Terpenoids. Latex, milky and sticky secretion form Calotropis procera has performed antimicrobial action against certain bacteria. The latex of Calotropis procera responds positively to Amylase assay. It displays the free space.The sample's concentration of each phytocompound is measured, and latex exhibits antibacterial actiontowards microorganisms. The growth of bacteria is prevented. The Lowry method and SDS-PAGE were also used for protein estimation and separation.
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References
Batista JS, De Lima JM, Freitas FJ, Amorim RN, Camara AC. (2011) Clinical and pathological effects of Calotropis procera exposure in sheep and rats. Toxicon 57:183– 185
Biedner B, Rothkoff L, Witztum A. (1977) Calotropis procera (Sodom apple) latex keratoconjunctivitis. Isr J Med Sci. 13:914-6
Barka Darra Wadhwani, a Deepak Mali, a Pooja Vyas, a Rashmy Nair b and Poonam Khandelwal (2021) A review on phytochemical constituents and pharmacological potential of Calotropis procera a Department of Chemistry, Mohanlal Sukhadia University, Udaipur313001, India. Department of Chemistry, S. S. Jain Subodh P.G. College, Jaipur-302004, India.
Basak S, Bahumik A, Mohanta A, Singhal P. (2019) Ocular toxicity by latex of Calotropis procera (Sodom apple). Indian J Ophthalmol: 57:232-234
C. Kaur, H.C. Kapoor (2002) Anti-oxidant activity and total phenolic content of some Asian vegetables Int. J. Food Sci. Technol., 37: 153-161
Geetha T. S. and Geetha N. (2014), Phytochemical Screening, Quantitative Analysis of Primary and Secondary Metabolites of Cymbopogancitratus (DC) stapf. leaves from Kodaikanal hills, Tamilnadu. International Journal of Pharm Tech Research. 6(2); 521-529.
Kumar A, Singh N and Kumar O (2019) Purification and Characterizations of Anti-Microbial Protein from Calotropis procera. Int. J. Curr. 30 Microbial. App. Sci (2019) pp. 1754-1761.
Kuriachen PM, Dave Y. (1989) Structural, developmental and histochemical studies in the collectors of Calotropis procera (Asclepiadaceae). J Phytological Res 2:7-14.
Lowry OH, Rosebraugh NJ, Farr AL, Randall RJ (1951) Protein Measurement with the Folin Phenol Reagent. Journal of Biological Chemistry 193: 265-275.
Lima-Filho JV, Patriota JM, Silva AF, Filho NT, Oliveira RS, et al. (2010) Proteins from latex of Calotropis procera prevent septic shock due to lethal infection by Salmonella enterica serovar Typhimurium. J Ethnopharmacol 129:327–334
Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680-5. doi: 10.1038/227680a0. PMID: 5432063.
Mohamed NH, Ismail MA, Abdel-Mageed WM, Shoreit AAM (2017) Biodegradation of Natural Rubber Latex of Calotropis procera by Two Endophytic Fungal Species. J BioremediatBiodegrad 8: 380. doi: 10.4172/2155-6199.1000380
Malik b, Shoib A. Baba a, Shahid A. (2015) Determination of total phenolic and flavonoid content, antimicrobial and antioxidant activity of a root extract of Arisaema jacquemontii Blume. Journal of Taibah University for Science 9: 449–454
Magalhaes HI, Ferreira PM, Moura ES, Torres MR, Alves AP, Pessoa OD. (2010). In vitro and in vivo antiproliferative activity of Calotropis procera stem extracts. An Acad Bras Cienc 82:407 16.
Mohammed A. M. Al-Qahtani, Mohammad Abul Farah, Faisal M. Abou-Tarboush, Khalid M. Al-Anazi, Naif O. Al-Harbi, Mohammad Ajmal Ali, Waleed A. Q. Hailan. (2020) Anticancer Effects of Calotropis procera Latex Extract in MCF-7 Breast Cancer Cells. Phcog Mag 2020; 16:550-6
Mohammad H. A. Hassan, Mady A. Ismail, Ahmed M. Moharram and Ahmed A. M. Shoreit (2017) Phytochemical and Antimicrobial of Latex Serum of Calotropis Procera and its Silver Nanoparticles Against Some Reference Pathogenic Strains J. Eco. Heal. Env. 5, No. 3, 65-75
Newman R A, Kondo Y, Yokoyama T, Dixon S, Cartwright C, Chan D. 2007 Autophagic cell death of human pancreatic tumor cells mediated by oleandrin, a lipid soluble cardiac glycoside. Integr Cancer Ther: 6:354 64
Ramos MV, Aguiar VC, Melo VM, Mesquita RO, Silvestre PP. 2007 Immunological and allergenic responses induced by latex fractions of Calotropis procera (Ait). R Br Journal of Ethnopharmacology 111: 115-122.
Reddy et al., 2008 Laboratory experiment in microbiology, Himalaya Publication House, 3rd edition.
Sharma S, Kumari A, Sharma M. 2016, Comparative GC-MS Analysis of Bioactive Compounds in Methanolic Extract of Calotropis gigantea (L) W.T. Aiton Leaf and Latex. International Journal of Pharmacognosy and Phytochemical Research. 8(11); 1823-1827.
Sumner J, Graham VA (1921) Dinitrosalicylic acid: a reagent for the estimation of sugar in normal and diabetic urine. J Biol Chem 47:5–9
Wang Y, Qiu Q, Shen JJ, Li DD, Jiang XJ, Si SY. (2012) Cardiac glycosides induce autophagy in human non small cell lung cancer cells through regulation of dual signaling pathways. Int J Biochem Cell Biol: 44:1813 24