Maximizing Sustainability: Leveraging Indigenous Fungal Cellulases for Sugarcane Bagasse Bioethanol Production and Agricultural Waste Management in Kano

Abstract

This study aimed to investigate the potential of locally isolated cellulolytic fungi in the production of bioethanol from sugarcane bagasse. The research encompassed the identification of effective fungal isolates, the stimulation of cellulase production, the characterization and purification of these cellulases, and the assessment of their efficiency in the hydrolysis of pre-treated sugarcane bagasse to facilitate ethanol production via the Simultaneous Saccharification and Fermentation (SSF) process. In the pursuit of the most effective isolate, cellulolytic fungi were isolated from soil samples and subjected to a rigorous screening process. Subsequently, the chosen fungus was cultivated in an environment rich in cellulose to promote cellulase production.The purified cellulases exhibited a noteworthy enzymatic activity, measuring 0.108 FPU/ml. These meticulously purified cellulases were subsequently harnessed for the enzymatic depolymerization of pre-treated sugarcane bagasse. This process culminated in the generation of a hydrolysate instrumental in ethanol production via SSF, in conjunction with Saccharomyces cerevisiae.This study underscores the feasibility of utilizing locally isolated fungal cellulases to transform pre-treated sugarcane bagasse into a viable bioethanol feedstock. This study highlights the conversion of abundant agricultural waste, specifically sugarcane bagasse, into valuable bioethanol by leveraging cellulases from locally isolated fungi. This eco-friendly approach not only addresses waste management challenges but also provides a renewable and sustainable energy source. Particularly beneficial in regions with substantial sugarcane bagasse disposal, this research promotes cost-effective waste utilization and sustainable energy production