Impact Of Pcb On Kidney Injury Molecules -1 & Regucalcin Mrna Expression In The Kidney And Beneficial Role Of Antioxidant Vitamin C And E
DOI:
https://doi.org/10.53555/jaz.v42i02.5355Keywords:
..................Abstract
PCBs are global environmental contaminants that cause disruption of the endocrine system by increasing free radical production in humans and animals which will cause major disorders. Besides, the human body has several counteract mechanisms for oxidative stress by producing antioxidants - in situ or external supplements like vit C and E. This highlights the potential role of the antioxidants in preventing and repairing damages caused by oxidative stress.
Aim: The study was aimed at assessing the impact of PCB on kidney injury molecule-1 (KIM 1) and regucalcin mRNA expression in kidney tissue and also to study the effect of antioxidant vitamin C and E against PCB.
Method: For this study, Male albino wistar rats weighing 180-200 grams were used. Animals were divided into three groups i.e, control group, PCB induced group, PCB induced VitaminC and E treated group consisting of 6 animals each. At the end of treatment the total RNA isolation was done followed by quantification of RNA, cDNA- reverse transcriptase, Quantitative analysis-real time PCR was done to analyze the mRNA expression levels of KIM-1 and regucalcin. The triplicate analysis results of the experiments performed on control and treated rats were expressed as mean ± SEM. Results were analyzed statistically by one-way analysis of variance (ANOVA) and significant differences between the mean values were measured using Duncan's multiple range test using Graph Pad Prism version 5. The results with p<0.05 level were considered to be statistically significant.
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Copyright (c) 2021 Priyanka R, R. Gayathri, J.Selvaraj, V.Vishnu priya, Kavitha.S, Dr.R.Gayathri
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