Development of UV Spectrophotometric Method for Estimation of Barberin in Bulk and Pharmaceutical Dosage Form

Abstract

A simple, accurate, precise spectrophotometric method was developed for the estimation of barberin (BRN). The optimum condition for the analysis of the BRN was studied. BRN was subjected to stress degradation under different conditions like acidic, alkali, neutral, oxidation, photolytic, and thermal degradation as per recommended by the International Conference on Harmonization (ICH). The samples thus prepared were used for degradation studies by with the developed method. The lambda max i.e. absorption maxima found at 348 nm and calibration curve linear over the range of 0-40 µg /ml. The standard regression equation and correlation coefficient fond to be y = 0.02x - 0.0189 R² = 0.9982 respectively. % RSD found to be less than one. The accepted limits of accuracy (recovery) were found to be 97.75% and all observed data are within required range which indicates good recovery value. LOD and LOQ found to be 0.0529 µg /ml and 0.2167 µg /ml respectively by developed UV spectroscopic method.