Assessment of Acute Toxicity, Oxygen Consumption, and Behavioral Changes in Channa Punctatus Exposed to Phorate 10% CG

Abstract

This study aimed to assess the toxicity of Phorate 10% CG on Channa punctatus using the Static and Flow-Through method for exposure durations of 24, 48, 72, and 96 hours. The study focused on monitoring mortality rates, evaluating oxygen consumption levels, and observing behavioral changes in response to Phorate exposure. Results showed a significant increase in Channa punctatus mortality rates with higher Phorate concentrations. The calculated LC50 values for the flow-through method at 24, 48, 72, and 96 hours were 0.572 mg/L, 0.550 mg/L, 0.500 mg/L, and 0.425 mg/L, respectively. This version ensures consistent formatting and proper scientific presentation. Fish were exposed to lethal and sub-lethal concentrations (1/10th of the 96-hour LC50 value-cfm) for 96 hours. Moreover, a negative correlation was found between Phorate concentration and oxygen consumption in Channa punctatus, indicating potential respiratory challenges caused by the pesticide. Behavioral changes observed in the study suggested pesticide-induced stress in the fish species. These results highlight the deleterious effects of Phorate on aquatic organisms and underscore the importance of monitoring mortality rates, oxygen consumption, and behavioral changes in toxicity assessments.